Regulatory
Part:BBa_K2116025:Design
Designed by: Asli Azizoglu Group: iGEM16_ETH_Zurich (2016-10-14)
EsaR repressible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The esabox has to be placed after the transcription start site to be able to act as a roadblock.
Source
The Anderson promoter sequences were taken form the registry. Esabox sequence comes from the Pantoea stewartii ssp. stewartii.